Application
Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 μg/ml.Application
Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 μg/ml.Application
Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | SLC1A7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Solute carrier family 1 (glutamate transporter) member 7, AAAT, excitatory amino acid transporter 5, EAAT5 |
| Product description | The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1). More recent evidence indicates that is it widely expressed in peripheral tissues, also (2). Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3). In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4).1) Arriza JL, Eliasof S, Kavanaugh MP, et al. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. USA 1997; 94:4155-60. |
| Immunogen | SLC1A7 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLC1A7. The immunogen is located within amino acids 460 - 510 of SLC1A7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC1A7 |
|---|---|
| Alternative Names | Solute carrier family 1 (glutamate transporter) member 7 AAAT excitatory amino acid transporter 5 EAAT5 |
| Molecular Weight(MW) | Predicted: 17, 52, 62, 68 kDa Observed: 52 kDa |
| Tissue Specificity | SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist. |
| Entrez Gene | 6512 |
|---|---|
| Protein Accession | NP_001274524 |
Application
Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 μg/ml.
Application
Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 μg/ml.
Application
Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1285 - C2C12 Cell Lysate |
|---|---|
| Application Notes | SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SLC1A7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SLC1A7 antibody is supplied in PBS containing 0.02% sodium azide. |
