SLC25A11 catalyzes the transport of 2-oxoglutarate across the inner mitochondrial membrane in an electroneutral exchange for malate or other dicarboxylic acids, and plays an important role in several metabolic processes, including the malate-aspartate shuttle, the oxoglutarate/isocitrate shuttle, in gluconeogenesis from lactate, and in nitrogen metabolism.1) Kabe, Y., (2006) J. Biol. Chem. 281 (42), 31729-31735.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SLC25A11.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
SLC25A11 antibody can be used for detection of SLC25A11 by ELISA at 1:1562500. SLC25A11 antibody can be used for detection of SLC25A11 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
SLC25A11 antibody can be used for detection of SLC25A11 by ELISA at 1:1562500. SLC25A11 antibody can be used for detection of SLC25A11 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SLC25A11 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.