Mitochondrial ADP/ATP carriers, such as SLC25A31, are nuclear-coded mitochondrial proteins that catalyze the exchange of ATP generated in mitochondria by ATP synthase (see MIM 108729) against ADP produced in cytosol by most energy-consuming reactions.Mitochondrial ADP/ATP carriers, such as SLC25A31, are nuclear-coded mitochondrial proteins that catalyze the exchange of ATP generated in mitochondria by ATP synthase (see MIM 108729) against ADP produced in cytosol by most energy-consuming reactions (Dolce et al., 2005 [PubMed 15670820]).1) Kim, Y.H., (2007) Dev. Biol. 302 (2), 463-476.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SLC25A31.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SLC25A31 antibody can be used for detection of SLC25A31 by ELISA at 1:62500. SLC25A31 antibody can be used for detection of SLC25A31 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
SLC25A31 antibody can be used for detection of SLC25A31 by ELISA at 1:62500. SLC25A31 antibody can be used for detection of SLC25A31 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SLC25A31 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.