Application
Immunofluorescence of SLC29A3 in rat colon muscle tissue with SLC29A3 antibody at 20 μg/mL.Application
Immunohistochemistry of SLC29A3 in rat colon tissue with SLC29A3 antibody at 5 μg/mL.Application
Western blot analysis of SLC29A3 in mouse bladder tissue lysate with SLC29A3 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | SLC29A3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Solute carrier family 29 member 3, Equilibrative nucleoside transporter 3, ENT3, HCLAP, HJCD, PHID |
| Product description | SLC29A3 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A3 is a transmembrane glycoprotein that localizes to the lysosomal membrane and is a broad selectivity, low affinity nucleoside transporter (3). Mutations in the SLC29A3 gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism (4). A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus (5).1) Hyde RJ, Cass CE, Young JD, et al. The ENT family of eukaryotic nucleoside and nucleobase transporters: recent advances in the investigation of structure/function relationships and the identification of novel isoforms. Mol. Membr. Biol. 2001; 18:53-63. |
| Immunogen | SLC29A3 antibody was raised against a 19 amino acid peptide near the amino terminus of human SLC29A3. The immunogen is located within the first 50 amino acids of SLC29A3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SLC29A3 antibody can be used for detection of SLC29A3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC29A3 |
|---|---|
| Alternative Names | Solute carrier family 29 member 3 Equilibrative nucleoside transporter 3 ENT3 HCLAP HJCD PHID |
| Molecular Weight(MW) | Predicted: 52 kDa Observed: 51 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (95%) |
| Tissue Specificity | SLC29A3 antibody is human, mouse and rat reactive. At least two isoforms of SLC29A3 are known to exist; this antibody will detect both isoforms. SLC29A3 antibody is predicted to not cross-react with other SLC29 proteins. |
Application
Immunofluorescence of SLC29A3 in rat colon muscle tissue with SLC29A3 antibody at 20 μg/mL.
Application
Immunohistochemistry of SLC29A3 in rat colon tissue with SLC29A3 antibody at 5 μg/mL.
Application
Western blot analysis of SLC29A3 in mouse bladder tissue lysate with SLC29A3 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1410 - Mouse Bladder Tissue Lysate |
|---|---|
| Application Notes | SLC29A3 antibody can be used for detection of SLC29A3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SLC29A3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SLC29A3 antibody is supplied in PBS containing 0.02% sodium azide. |
