Application
Western blot analysis of SLC29A4 in SK-N-SH cell lysate with SLC29A4 antibody at 1 μg/ml.Application
Immunofluorescence of SLC29A4 in human brain tissue with SLC29A4 antibody at 20 μg/mL.Application
Immunohistochemistry of SLC29A4 in human brain tissue with SLC29A4 antibody at 5 μg/mL.| Product Name | SLC29A4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Solute carrier family 29 member 4, Equilibrative nucleoside transporter 4, ENT4, PMAT |
| Product description | SLC29A4 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A4 is a transmembrane protein that catalyzes the reuptake of monoamines into presynaptic neurons, thus determining the intensity and duration of monoamine neural signaling (1,3). SLC29A4 has been shown to transport several compounds, including serotonin, dopamine, and the neurotoxin 1-methyl-4-phenylpyridinium (3).1) Engel K, Zhou M, and Wang J. Identification and characterization of a novel monomine transporter in the human brain. J. Biol. Chem. 2004; 279:50042-9. |
| Immunogen | SLC29A4 antibody was raised against an 18 amino acid peptide near the center of human SLC29A4. The immunogen is located within amino acids 310 - 360 of SLC29A4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SLC29A4 antibody can be used for detection of SLC29A4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC29A4 |
|---|---|
| Alternative Names | Solute carrier family 29 member 4 Equilibrative nucleoside transporter 4 ENT4 PMAT |
| Molecular Weight(MW) | Predicted: 58 kDa Observed: 58 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | SLC29A4 antibody is human specific. SLC29A4 antibody is predicted to not cross-react with other SLC29 proteins. |
| Entrez Gene | 222962 |
|---|---|
| Protein Accession | NP_001035751 |
Application
Western blot analysis of SLC29A4 in SK-N-SH cell lysate with SLC29A4 antibody at 1 μg/ml.
Application
Immunofluorescence of SLC29A4 in human brain tissue with SLC29A4 antibody at 20 μg/mL.
Application
Immunohistochemistry of SLC29A4 in human brain tissue with SLC29A4 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | SLC29A4 antibody can be used for detection of SLC29A4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SLC29A4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SLC29A4 antibody is supplied in PBS containing 0.02% sodium azide. |
