Application
Immunocytochemistry of SLC35D2 in HeLa cells with SLC35D2 antibody at 5 μg/mL.Application
Western blot analysis of SLC35D2 in HeLa cell lysate with SLC35D2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SLC35D2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC35D2 Antibody: hfrc, HFRC1, SQV7L, UGTrel8, HFRC, UGTREL8, Homolog of Fringe connection protein 1 |
| Product description | SLC35D2 Antibody: The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident SLC family member SLC35D2 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. Its overexpression in transfected cells modulated cell surface heparin sulfate expression, suggesting that SLC35D2 is involved in heparin sulfate synthesis. SLC35D2-overexpressing cells also showed increased constitutive and hypotonic stress-stimulated release of UDP-GlcNAc, suggesting that SLC35D2 may be involved in UDP-sugar release and cell signaling.1) Ishida N and Kawakita M. Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35). Pflugers Arch. 2004; 447:768-75. |
| Immunogen | SLC35D2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SLC35D2. The immunogen is located within the last 50 amino acids of SLC35D2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| SLC35D2 antibody can be used for detection of SLC35D2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC35D2 |
|---|---|
| Alternative Names | SLC35D2 Antibody: hfrc HFRC1 SQV7L UGTrel8 HFRC UGTREL8 Homolog of Fringe connection protein 1 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least two isoforms of SLC35D2 are known to exist; this antibody will recognize both isoforms. SLC35D2 antibody is predicted to not cross-react with SLC35D1 or SLC35D3. |
Application
Immunocytochemistry of SLC35D2 in HeLa cells with SLC35D2 antibody at 5 μg/mL.
Application
Western blot analysis of SLC35D2 in HeLa cell lysate with SLC35D2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | SLC35D2 antibody can be used for detection of SLC35D2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SLC35D2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SLC35D2 Antibody is supplied in PBS containing 0.02% sodium azide. |
