Application
Immunocytochemistry of SLCO1B1 in HepG2 cells with SLCO1B1 antibody at 2.5 μg/ml.Application
Western blot analysis of SLCO1B1 in human liver tissue lysate with SLCO1B1 antibody at 1 μg/ml.Application
Immunofluorescence of SLCO1B1 in human liver tissue with SLCO1B1 antibody at 20 μg/ml.| Product Name | SLCO1B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Solute carrier organic anion transporter family member 1B1, LST1, Solute carrier family 21 member 6, SLC21A6, Sodium-independent organic anion-transporting polypeptide 2, OATP1B1, OATP2, OATPC, Solute carrier family 21 member 6 |
| Product description | SLCO1B1 is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and may play an important role in the clearance of bile acids and organic anions from the liver (1,2). It contains one Kazal-like domain and belongs to the organo-anion transporter family (2,3). SLCO1B1 is highly expressed in liver and is localized to the basolateral hepatocyte membrane. It is responsible for the hepatic uptake of the liver-specific hydroxymethylglutaryl-CoA reductase inhibitor in mouse, rat and human (3,4).1) Abe T, Kakyo M, Tokui T, et al. Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1. J. Biol. Chem. 1999; 274:17159-63. |
| Immunogen | SLCO1B1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLCO1B1. The immunogen is located within the last 50 amino acids of SLCO1B1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SLCO1B1 antibody can be used for detection of SLCO1B1 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLCO1B1 |
|---|---|
| Alternative Names | Solute carrier organic anion transporter family member 1B1 LST1 Solute carrier family 21 member 6 SLC21A6 Sodium-independent organic anion-transporting polypeptide 2 OATP1B1 OATP2 OATPC Solute carrier family 21 member 6 |
| Molecular Weight(MW) | Predicted: 76 kDa Observed: 75 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | SLCO1B1 antibody is human specific. At least four isoforms of SLCO1B1 are known to exist. This antibody is predicted to not cross-react with other SLCO1B proteins. |
Application
Immunocytochemistry of SLCO1B1 in HepG2 cells with SLCO1B1 antibody at 2.5 μg/ml.
Application
Western blot analysis of SLCO1B1 in human liver tissue lysate with SLCO1B1 antibody at 1 μg/ml.
Application
Immunofluorescence of SLCO1B1 in human liver tissue with SLCO1B1 antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | SLCO1B1 antibody can be used for detection of SLCO1B1 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SLCO1B1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SLCO1B1 antibody is supplied in PBS containing 0.02% sodium azide. |
