Application
Immunohistochemistry of Slitrk2 in human brain tissue with Slitrk2 antibody at 2.5 μg/mL.Application
Immunofluorescence of Slitrk2 in Human Brain cells with Slitrk2 antibody at 20 μg/mL.Application
Western blot analysis of Slitrk2 in rat brain tissue lysate with Slitrk2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Slitrk2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Slitrk2 Antibody: CXorf2, SLITL1, CXorf2, KIAA1854, UNQ9197/PRO34756, SLIT and NTRK-like protein 2 |
| Product description | Slitrk2 Antibody: SLIT and NTRK-like family 2 (Slitrk2) is a member a protein family consisting of six homologous transmembrane proteins (Slitrk1-6) that share two conserved leucine-rich repeat domains in the extracellular domain and have significant homology to Slit, a secreted axonal growth-controlling protein. These proteins are also homologous to trk neurotrophin receptors in their intracellular domains. Expression of Slitrk proteins is highly restricted to neural and brain tumor tissues, but varies within the family. For example, Slitrk2 is expressed primarily in neurons in the ventricular layer of the brain. Like every other Slitrk protein except Slitrk1, overexpression of Slitrk2 inhibited neurite outgrowth in cultured neurons. Deletion analysis indicated that the functional difference between Slitrk2 and Slitrk1 is within their intracellular domains. At least two isoforms of this protein are known to exist.1) Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29. |
| Immunogen | Slitrk2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human Slitrk2. The immunogen is located within amino acids 770 - 820 of Slitrk2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Slitrk2 antibody can be used for detection of Slitrk2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLITRK2 |
|---|---|
| Alternative Names | Slitrk2 Antibody: CXorf2 SLITL1 CXorf2 KIAA1854 UNQ9197/PRO34756 SLIT and NTRK-like protein 2 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to other Slitrk proteins. |
Application
Immunohistochemistry of Slitrk2 in human brain tissue with Slitrk2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of Slitrk2 in Human Brain cells with Slitrk2 antibody at 20 μg/mL.
Application
Western blot analysis of Slitrk2 in rat brain tissue lysate with Slitrk2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
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| Application Notes | Slitrk2 antibody can be used for detection of Slitrk2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Slitrk2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Slitrk2 Antibody is supplied in PBS containing 0.02% sodium azide. |
