Application
Immunohistochemistry of Slitrk3 in human brain tissue with Slitrk3 antibody at 2.5 μg/mL.Application
Immunofluorescence of slitrk3 in human brain tissue with slitrk3 antibody at 20 μg/mL.Application
Western blot analysis of Slitrk3 in SK-N-SH cell lysate with Slitrk3 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | Slitrk3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Slitrk3 Antibody: KIAA0848, SLIT and NTRK-like protein 3 |
| Product description | Slitrk3 Antibody: SLIT and NTRK-like family 3 (Slitrk3) is a member a protein family consisting of six homologous transmembrane proteins (Slitrk1-6) that share two conserved leucine-rich repeat domains in the extracellular domain and have significant homology to Slit, a secreted axonal growth-controlling protein. These proteins are also homologous to trk neurotrophin receptors in their intracellular domains. Expression of Slitrk proteins is highly restricted to neural and brain tumor tissues, but varies within the protein family. Like every other Slitrk protein except Slitrk1, overexpression of Slitrk3 inhibited neurite outgrowth in cultured neurons, suggesting that these proteins are involved in the control of neurite outgrowth.1) Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29. |
| Immunogen | Slitrk3 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Slitrk3. The immunogen is located within amino acids 320 - 370 of Slitrk3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Slitrk3 antibody can be used for detection of Slitrk3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLITRK3 |
|---|---|
| Alternative Names | Slitrk3 Antibody: KIAA0848 SLIT and NTRK-like protein 3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to other Slitrk proteins. |
Application
Immunohistochemistry of Slitrk3 in human brain tissue with Slitrk3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of slitrk3 in human brain tissue with slitrk3 antibody at 20 μg/mL.
Application
Western blot analysis of Slitrk3 in SK-N-SH cell lysate with Slitrk3 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | Slitrk3 antibody can be used for detection of Slitrk3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Slitrk3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Slitrk3 Antibody is supplied in PBS containing 0.02% sodium azide. |
