Application
Immunohistochemistry of Slitrk4 in human brain tissue with Slitrk4 antibody at 2.5 μg/mL.Application
Immunofluorescence of slitrk4 in human brain tissue with slitrk4 antibody at 20 μg/mL.Application
Western blot analysis of Slitrk4 in mouse brain tissue lysate with Slitrk4 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | Slitrk4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Slitrk4 Antibody: SLIT and NTRK-like protein 4 |
| Product description | Slitrk4 Antibody: SLIT and NTRK-like family 4 (Slitrk4) is a member a protein family consisting of six homologous transmembrane proteins (Slitrk1-6) that share two conserved leucine-rich repeat domains in the extracellular domain and have significant homology to Slit, a secreted axonal growth-controlling protein. These proteins are also homologous to trk neurotrophin receptors in their intracellular domains. Expression of Slitrk proteins is highly restricted to neural and brain tumor tissues, but varies within the protein family. Like every other Slitrk protein except Slitrk1, overexpression of Slitrk4 inhibited neurite outgrowth in cultured neurons, suggesting that these proteins are involved in the control of neurite outgrowth.1) Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29. |
| Immunogen | Slitrk4 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Slitrk4. The immunogen is located within amino acids 30 - 80 of Slitrk4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Slitrk4 antibody can be used for detection of Slitrk4 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLITRK4 |
|---|---|
| Alternative Names | Slitrk4 Antibody: SLIT and NTRK-like protein 4 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to other Slitrk proteins. |
Application
Immunohistochemistry of Slitrk4 in human brain tissue with Slitrk4 antibody at 2.5 μg/mL.
Application
Immunofluorescence of slitrk4 in human brain tissue with slitrk4 antibody at 20 μg/mL.
Application
Western blot analysis of Slitrk4 in mouse brain tissue lysate with Slitrk4 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | Slitrk4 antibody can be used for detection of Slitrk4 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Slitrk4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Slitrk4 Antibody is supplied in PBS containing 0.02% sodium azide. |
