Application
Immunohistochemistry of Slitrk4 in human brain tissue with Slitrk4 antibody at 2.5 μg/mL.Application
Western blot analysis of Slitrk5 in 3T3 cell lysate with Slitrk5 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.Application
Immunofluorescence of Slitrk5 in Human Brain cells with Slitrk5 antibody at 5 μg/mL.| Product Name | Slitrk5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Slitrk5 Antibody: LRRC11, bA364G4.2, KIAA0918, LRRC11, SLIT and NTRK-like protein 5, Leucine-rich repeat-containing protein 11 |
| Product description | Slitrk5 Antibody: SLIT and NTRK-like family 5 (Slitrk5) is a member a protein family consisting of six homologous transmembrane proteins (Slitrk1-6) that share two conserved leucine-rich repeat domains in the extracellular domain and have significant homology to Slit, a secreted axonal growth-controlling protein. These proteins are also homologous to trk neurotrophin receptors in their intracellular domains. Expression of Slitrk proteins is highly restricted to neural and brain tumor tissues, but varies within the protein family. Like every other Slitrk protein except Slitrk1, overexpression of Slitrk5 inhibited neurite outgrowth in cultured neurons, suggesting that these proteins are involved in the control of neurite outgrowth.1) Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29. |
| Immunogen | Slitrk5 antibody was raised against a 14 amino acid synthetic peptide from near the center of human Slitrk5. The immunogen is located within amino acids 310 - 360 of Slitrk5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Slitrk5 antibody can be used for detection of Slitrk5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLITRK5 |
|---|---|
| Alternative Names | Slitrk5 Antibody: LRRC11 bA364G4.2 KIAA0918 LRRC11 SLIT and NTRK-like protein 5 Leucine-rich repeat-containing protein 11 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to other Slitrk proteins. |
Application
Immunohistochemistry of Slitrk4 in human brain tissue with Slitrk4 antibody at 2.5 μg/mL.
Application
Western blot analysis of Slitrk5 in 3T3 cell lysate with Slitrk5 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.
Application
Immunofluorescence of Slitrk5 in Human Brain cells with Slitrk5 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | Slitrk5 antibody can be used for detection of Slitrk5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Slitrk5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Slitrk5 Antibody is supplied in PBS containing 0.02% sodium azide. |
