Application
Western blot analysis of extracts from HeLa cells treated with TNF using Phospho-Smad3 (Thr179) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Smad3 (phospho Thr179) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.1) Cohen-Solal KA, et al. (2011) Pigment Cell Melanoma Res 24, 512-24 |
| Immunogen | Smad3 (phospho Thr179) antibody was raised against a peptide sequence around phosphorylation site of threonine179 (P-E-T (p)-P-P) derived from Human Smad3 . |
| Modification | p-Thr179 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD3 |
|---|---|
| Alternative Names | JV15-2 MAD-3 MADH3 Smad 3 Mothers against decapentaplegic homolog 3 |
| Molecular Weight(MW) | 50 kDa |
| Entrez Gene | 4088 |
|---|---|
| Protein Accession | NP_001138574.1 |
Application
Western blot analysis of extracts from HeLa cells treated with TNF using Phospho-Smad3 (Thr179) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
