Application
Antibody used in WB on Human U2OS.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in WB on Human U2OS at 1:1000.| Product Name | SMAD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD4, JIP, DPC4, MADH4, MYHRS |
| Product description | SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction1) Kantola, A.K., et al., Gene 363, 142-150 (2005). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMAD4 |
|---|---|
| Alternative Names | SMAD4 JIP DPC4 MADH4 MYHRS |
| Molecular Weight(MW) | 60 kDa |
Application
Antibody used in WB on Human U2OS.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in WB on Human U2OS at 1:1000.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
