Application
Confocal immunofluorescent analysis of SMAD4 Antibody (T277) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of SMAD4 Antibody (T277) using rabbit polyclonal SMAD4 Antibody (T277) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).| Product Name | SMAD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.1) Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004). |
| Immunogen | This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD4 |
|---|---|
| Alternative Names | Mothers against decapentaplegic homolog 4 MAD homolog 4 Mothers against DPP homolog 4 Deletion target in pancreatic carcinoma 4 SMAD family member 4 SMAD 4 Smad4 hSMAD4 SMAD4 DPC4 MADH4 |
| Molecular Weight(MW) | 60 kDa |
Application
Confocal immunofluorescent analysis of SMAD4 Antibody (T277) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of SMAD4 Antibody (T277) using rabbit polyclonal SMAD4 Antibody (T277) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
