Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Product Name | SMAD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD5, DWFC, JV5-1, MADH5 |
| Product description | SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.1) Langenfeld, E.M., et al., (2006) Oncogene 25 (5), 685-692. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMAD5 |
|---|---|
| Alternative Names | SMAD5 DWFC JV5-1 MADH5 |
| Molecular Weight(MW) | 51 kDa, 52 kDa, 52 kDa |
| Entrez Gene | 4090 |
|---|---|
| Protein Accession | NP_001001420 |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
