Application
Antibody used in WB on Transfected 293 extracts at 1:500.Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Product Name | SMAD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.1) Komuro, A., et al., (2004) Oncogene 23 (41), 6914-6923. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:12500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD7 |
|---|---|
| Alternative Names | SMAD7 CRCS3 MADH7 MADH8 |
| Molecular Weight(MW) | 46 kDa |
Application
Antibody used in WB on Transfected 293 extracts at 1:500.
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:12500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
