Application
Fluorescent confocal image of A431 cell stained with Smad7 Antibody . A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).Smad7 immunoreactivApplication
Smad7 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of Smad7 Antibody in ZR-75-1 cell line lysates (35ug/lane)| Product Name | Smad7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Smad7 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta super family.1) Hirata,H., et.al., Cancer 115 (19), 4488-4503 (2009) |
| Immunogen | This Smad7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the Central region of human Smad7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SMAD7 |
|---|---|
| Alternative Names | Mothers against decapentaplegic homolog 7 MAD homolog 7 Mothers against DPP homolog 7 Mothers against decapentaplegic homolog 8 MAD homolog 8 Mothers against DPP homolog 8 SMAD family member 7 SMAD 7 Smad7 hSMAD7 SMAD7 MADH7 MADH8 |
| Molecular Weight(MW) | 46 kDa |
Application
Fluorescent confocal image of A431 cell stained with Smad7 Antibody . A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).Smad7 immunoreactiv
Application
Smad7 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of Smad7 Antibody in ZR-75-1 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
