Application
Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody . MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloApplication
Western blot analysis in A549 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | SMAD9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD9 |
|---|---|
| Alternative Names | Mothers against decapentaplegic homolog 9 MAD homolog 9 Mothers against DPP homolog 9 Madh6 SMAD family member 9 SMAD 9 Smad9 SMAD9 MADH6 MADH9 |
| Molecular Weight(MW) | 52 kDa |
Application
Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody . MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phallo
Application
Western blot analysis in A549 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
