cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN.1) Okazaki, Y., et al., (2002) Nature 420 (6915), 563-573.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SMARCA1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SMARCA1 antibody can be used for detection of SMARCA1 by ELISA at 1:312500. SMARCA1 antibody can be used for detection of SMARCA1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
SMARCA1 antibody can be used for detection of SMARCA1 by ELISA at 1:312500. SMARCA1 antibody can be used for detection of SMARCA1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMARCA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt