Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Product Name | SMPD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMPD1 is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB).1) Zeidan, Y.H., (2008) J. Cell Biol. 181 (2), 335-350. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMPD1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SMPD1 antibody can be used for detection of SMPD1 by ELISA at 1:62500. SMPD1 antibody can be used for detection of SMPD1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMPD1 |
|---|---|
| Alternative Names | SMPD1 ASM NPD ASMASE |
| Molecular Weight(MW) | 65 kDa |
Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | SMPD1 antibody can be used for detection of SMPD1 by ELISA at 1:62500. SMPD1 antibody can be used for detection of SMPD1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMPD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
