WB
Western blot analysis of lysates from wild type (WT) and SMPD4 knockout (KO) HeLa cells,using SMPD1Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.WB
Western blot analysis of lysates from Rat liver using SMPD1 Rabbit mAbat 1:1000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffinembedded Human brain using [KO Validated]SMPD1 Rabbit mAb at dilution of1:100 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.IF-F
Confocal imaging of frozen sections Rat livertissue using [KO Validated] SMPD1 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3-conjugated Goatanti-Rabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining(Blue). Microwave antigen retrievalperformed with 0.01M Citrate Buffer (pH 6.0)prior to IF staining. Objective: 40x.| Product Name | [KO Validated] SMPD1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IF-F:1:100-1:400 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMPD1 |
|---|---|
| Gene Synonyms | ASM NPD ASMASE |
| Gene Full Name | sphingomyelin phosphodiesterase 1 |
| Gene Summary | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] |
| Alternative Names | ASM NPD ASMASE D1 |
| Molecular Weight(MW) | 70kDa(Observed band size:70kDa) |
| Cellular Localization | Lysosome. |
WB
Western blot analysis of lysates from wild type (WT) and SMPD4 knockout (KO) HeLa cells,using SMPD1Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
WB
Western blot analysis of lysates from Rat liver using SMPD1 Rabbit mAbat 1:1000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffinembedded Human brain using [KO Validated]SMPD1 Rabbit mAb at dilution of1:100 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.
IF-F
Confocal imaging of frozen sections Rat livertissue using [KO Validated] SMPD1 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3-conjugated Goatanti-Rabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining(Blue). Microwave antigen retrievalperformed with 0.01M Citrate Buffer (pH 6.0)prior to IF staining. Objective: 40x.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IF-F:1:100-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
