SNAPC1 is a part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. It binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes and recruits TBP and BRF2 to the U6 snRNA TATA box.1) Gu, L., (2005) J. Biol. Chem. 280 (30), 27697-27704.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SNAPC1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SNAPC1 antibody can be used for detection of SNAPC1 by ELISA at 1:312500. SNAPC1 antibody can be used for detection of SNAPC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
SNAPC1 antibody can be used for detection of SNAPC1 by ELISA at 1:312500. SNAPC1 antibody can be used for detection of SNAPC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SNAPC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt