Application
Western blot analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 μg/ml.Application
Immunohistochemistry of SNCA in rat brain tissue with SNCA antibody at 5 μg/ml.Application
Immunofluorescence of SNCA in rat brain tissue with SNCA antibody at 20 μg/ml.Application
Western blot analysis of SNCA in mouse cerebellum tissue lysate with SNCA antibody at 1 μg/ml.| Product Name | SNCA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Alpha-Synuclein (SNCA) is a hallmark of Alzheimer’s disease (1,2). It is a cytoplasmic protein that is predominantly expressed in the central nervous system (2). SNCA reduces neuronal responsiveness to various apoptotic stimuli, leading to the decreased caspase-3 activation. SNCA may be involved in the regulation of dopamine release and transport and induces fibrillization of microtubule-associated protein tau (3). Defects in SNCA are associated with familial Parkinson’s disease (4,5).1) Ueda K, Fukushima H, Masliah E, et al. Molecular cloning of cDNA encoding an unrecognized component of amyloid in Alzheimer disease. Proc. Natl. Acad. Sci. USA 1993; 90:11282-6. |
| Immunogen | SNCA antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SNCA. The immunogen is located within the last 50 amino acids of SNCA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SNCA antibody can be used for detection of SNCA by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNCA |
|---|---|
| Alternative Names | SNCA Antibody: PD1 NACP PARK1 PARK4 Alpha-synuclein Non-A beta component of AD amyloid |
| Molecular Weight(MW) | Predicted: 12 kDa Observed: 16 kDa |
Application
Western blot analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 μg/ml.
Application
Immunohistochemistry of SNCA in rat brain tissue with SNCA antibody at 5 μg/ml.
Application
Immunofluorescence of SNCA in rat brain tissue with SNCA antibody at 20 μg/ml.
Application
Western blot analysis of SNCA in mouse cerebellum tissue lysate with SNCA antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1412 - Mouse Cerebellum Tissue Lysate |
|---|---|
| Application Notes | SNCA antibody can be used for detection of SNCA by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SNCA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SNCA antibody is supplied in PBS containing 0.02% sodium azide. |
