Application
Western blot analysis using SNCG monoclonal antibody against truncated SNCG recombinant protein.Application
Immunohistochemical analysis of paraffin - embedded human ovary carcinoma (top) and breast carcinoma (bottom) showing cytoplasmic (ovary carcinoma) and cytoplasmic / nuclear (breast carcinoma) location using SNCG antibody with DAB staining.| Product Name | SNCG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SNCG(also designated gamma-synuclein or breast cancer-specific protein 1),with 127-amino acid protein(about 14 kDa), belongs to the synuclein family, which also includes alpha- and beta- synuclein.Three synucleins are located in the neuronal cytosol and enriched in presynaptic terminals,while SNCG is also expressed in many other non-neuronal tissues. SNCG is abnormally expressed in a high percentage of tumor tissues of diversified cancer types, including liver, esophagus, colon, gastric, lung, prostate, cervical, and breast cancer, but rarely expressed in tumor-matched nonneoplastic adjacent tissues. High levels of SNCG have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development.1) Haiyan Liu, Wei Liu, Yinwei Wu. Cancer Res. 2005 Sep 1;65(17):7635-43. |
| Immunogen | Ni-NTA purified truncated recombinant SNCG expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNCG |
|---|---|
| Alternative Names | BCSG1 PERSYN PRSN Breast cancer-specific gene 1 protein SR BCSG1 |
Application
Western blot analysis using SNCG monoclonal antibody against truncated SNCG recombinant protein.
Application
Immunohistochemical analysis of paraffin - embedded human ovary carcinoma (top) and breast carcinoma (bottom) showing cytoplasmic (ovary carcinoma) and cytoplasmic / nuclear (breast carcinoma) location using SNCG antibody with DAB staining.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SNCG (breast cancer-specific protein 1) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
