Application
Antibody used in IHC on Human Stomach at 4.0-8.0 ug/ml.Application
Antibody used in IHC on Human Heart.Application
Antibody used in WB on Human HepG2 at 5.0 ug/ml.Application
Antibody used in IHC on Human Spleen.| Product Name | SNF8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia (AML). In addition to its elongation activity, ELL contains a novel type of RNA polymerase II interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. EAP30 is a subunit of the ELL complex. EAP30 can interact with ELL and derepress ELL's inhibitory activity in vitro.SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).1) Malerod, L., (2007) Traffic 8 (11), 1617-1629. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SNF8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SNF8 antibody can be used for detection of SNF8 by ELISA at 1:1562500. SNF8 antibody can be used for detection of SNF8 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNF8 |
|---|---|
| Alternative Names | SNF8 Dot3 EAP30 VPS22 |
| Molecular Weight(MW) | 29 kDa |
Application
Antibody used in IHC on Human Stomach at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human Heart.
Application
Antibody used in WB on Human HepG2 at 5.0 ug/ml.
Application
Antibody used in IHC on Human Spleen.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SNF8 antibody can be used for detection of SNF8 by ELISA at 1:1562500. SNF8 antibody can be used for detection of SNF8 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SNF8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
