Application
Western blot analysis of SNF8 using rabbit polyclonal SNF8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | SNF8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).1) Malerod, L., et al. Traffic 8(11):1617-1629(2007) |
| Immunogen | This SNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human SNF8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNF8 |
|---|---|
| Alternative Names | Vacuolar-sorting protein SNF8 ELL-associated protein of 30 kDa ESCRT-II complex subunit VPS22 hVps22 SNF8 EAP30 |
| Molecular Weight(MW) | 29 kDa |
Application
Western blot analysis of SNF8 using rabbit polyclonal SNF8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
