Application
Western Blot analysis of Somatic Cytochrome C inHeLa cell lysate (15 μg) using Somatic Cytochrome C monoclonal antibody (1:1000).Application
Immunohistochemistry staining of Somatic Cytochrome C in human bone marrow tissue using Somatic Cytochrome C Monoclonal Antibody.| Product Name | Somatic Cytochrome C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic |
| Product description | In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3. |
| Immunogen | Somatic Cytochrome C monoclonal antibody was raised against amino acids 1 - 80 81-104 and 66-104 of Somatic Cytochrome C. (Pigeon) |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| Somatic Cytochrome C antibody can be used in ELISA, immunohistochemistry starting at 5 μg/mL, immunocytochemistry, immunofluorescence, and immunoprecipitation.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CYCS |
|---|---|
| Alternative Names | CYCS CYC HCS THC4 Cytochrome c Cytochrome c somatic |
| Tissue Specificity | An approx. 15 kD band is observed. This recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached). |
Application
Western Blot analysis of Somatic Cytochrome C inHeLa cell lysate (15 μg) using Somatic Cytochrome C monoclonal antibody (1:1000).
Application
Immunohistochemistry staining of Somatic Cytochrome C in human bone marrow tissue using Somatic Cytochrome C Monoclonal Antibody.| Application Notes | Somatic Cytochrome C antibody can be used in ELISA, immunohistochemistry starting at 5 μg/mL, immunocytochemistry, immunofluorescence, and immunoprecipitation.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store Somatic Cytochrome C antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
