Application
Confocal immunofluorescent analysis of SORBS1 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in CHO cell line lysates (35ug/lane).Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Product Name | SORBS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Sorbin and SH3 domain-containing protein 1, Ponsin, SH3 domain protein 5, SH3P12, c-Cbl-associated protein, CAP, SORBS1 ( HGNC:14565 ) |
| Product description | Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity).1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010) Gehmlich, K., et al. Eur. J. Cell Biol. 89(5):351-364(2010) Yamada, Y., et al. Hypertens. Res. 32(5):325-331(2009) Fernow, I., et al. BMC Cell Biol. 10, 80 (2009) : |
| Immunogen | This SORBS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 866-892 amino acids from the Central region of human SORBS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HamsterHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SORBS1 |
|---|---|
| Alternative Names | Sorbin and SH3 domain-containing protein 1 Ponsin SH3 domain protein 5 SH3P12 c-Cbl-associated protein CAP SORBS1 ( HGNC:14565 ) |
| Molecular Weight(MW) | 143 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of SORBS1 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in CHO cell line lysates (35ug/lane).
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
