Application
Western blot analysis of SOX17 in HepG2 cell lysate with SOX17 antibody at (A) 0.5 and (B) 1 μg/ml.Application
Immunofluorescence of SOX17 in mouse liver tissue with SOX17 antibody at 20 μg/ml.| Product Name | SOX17 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOX17 Antibody: VUR3, Transcription factor SOX-17 |
| Product description | SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate (1,2). SOX17 is part of the SoxF subgroup which plays an important role in the differentiation of different cell types, including visceral and definitive endoderm and hematopoietic cell types (3,4). SOX17 is also essential for acquisition and maintenance of arterial identity (5).1) Kanai Y, Kanai-Azuma M, Noce T, et al. Identification of two Sox17 messenger RNA isoforms, with and without the high mobility group box region, and their differential expression in mouse spermatogenesis. J. Cell Biol. 1996; 133:667-81. |
| Immunogen | SOX17 antibody was raised against a 16 amino acid peptide near the center of human SOX17. The immunogen is located within amino acids 240 - 290 of SOX17. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SOX17 antibody can be used for detection of SOX17 by Western blot at 0.5 - 1 μg/ml. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOX17 |
|---|---|
| Alternative Names | SOX17 Antibody: VUR3 Transcription factor SOX-17 |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 42 kDa |
| Tissue Specificity | SOX17 antibody is human, mouse and rat reactive. SOX17 is predicted to not cross-react with other SOX proteins. |
Application
Western blot analysis of SOX17 in HepG2 cell lysate with SOX17 antibody at (A) 0.5 and (B) 1 μg/ml.
Application
Immunofluorescence of SOX17 in mouse liver tissue with SOX17 antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SOX17 antibody can be used for detection of SOX17 by Western blot at 0.5 - 1 μg/ml. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SOX17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SOX17 antibody is supplied in PBS containing 0.02% sodium azide. |
