Application Antibody used in WB on U87 at 1:1000.
| Product Name | SOX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Wang, P., (2008) Arch. Ophthalmol. 126 (5), 709-713 . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOX2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| SOX2 antibody can be used for detection of SOX2 by ELISA at 1:12500. SOX2 antibody can be used for detection of SOX2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOX2 |
|---|---|
| Alternative Names | SOX2 ANOP3 MCOPS3 MGC2413 |
| Molecular Weight(MW) | 34 kDa |
Application Antibody used in WB on U87 at 1:1000.
| Positive Control | 1) OVCAR-3 Cell Lysate |
|---|---|
| Application Notes | SOX2 antibody can be used for detection of SOX2 by ELISA at 1:12500. SOX2 antibody can be used for detection of SOX2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SOX2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
