Application
Antibody used in WB on HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.Application
Antibody used in WB on Human HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Product Name | SOX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOX5, L-SOX5, L-SOX5B, L-SOX5F, RP11-444D3.1 |
| Product description | SOX5 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8.1) Ikeda, T., et al., () Gene 298 (1), 59-68 2002. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOX5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SOX5 antibody can be used for detection of SOX5 by ELISA at 1:62500. SOX5 antibody can be used for detection of SOX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOX5 |
|---|---|
| Alternative Names | SOX5 L-SOX5 L-SOX5B L-SOX5F RP11-444D3.1 |
| Molecular Weight(MW) | 42 kDa |
Application
Antibody used in WB on HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SOX5 antibody can be used for detection of SOX5 by ELISA at 1:62500. SOX5 antibody can be used for detection of SOX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SOX5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
