SP140 is the nuclear body protein found specifically in all NP cells. HIV-1 infection induced its partial dispersal from nuclear bodies into cytosolic colocalization with Vif.1) Madani, N., et al., (2002) J. Virol. 76 (21), 11133-11138.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SP140.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
SP140 antibody can be used for detection of SP140 by ELISA at 1:62500. SP140 antibody can be used for detection of SP140 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Raji at 5.0 ug/ml.
Positive Control
1) Cat. No. 1207 - Raji Cell Lysate
Application Notes
SP140 antibody can be used for detection of SP140 by ELISA at 1:62500. SP140 antibody can be used for detection of SP140 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SP140 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt