Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Product Name | SPAG8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPAG8, BS-84, HSD-1, MGC26201, SMP1, SPAG3, hSMP-1, CT142, CILD28 |
| Product description | The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. SPAG8 is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.1) Cheng, G.Y., (2007) Asian J. Androl. 9 (1), 23-29. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPAG8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SPAG8 antibody can be used for detection of SPAG8 by ELISA at 1:312500. SPAG8 antibody can be used for detection of SPAG8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPAG8 |
|---|---|
| Alternative Names | SPAG8 BS-84 HSD-1 MGC26201 SMP1 SPAG3 hSMP-1 CT142 CILD28 |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | SPAG8 antibody can be used for detection of SPAG8 by ELISA at 1:312500. SPAG8 antibody can be used for detection of SPAG8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPAG8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
