Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | SPAG8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Sperm-associated antigen 8, HSD-1, Sperm membrane protein 1, SMP-1, Sperm membrane protein BS-84, SPAG8 |
| Product description | The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.1) Wu, H., et al. FEBS Lett. 584(13):2807-2815(2010) |
| Immunogen | This SPAG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-411 amino acids from the C-terminal region of human SPAG8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPAG8 |
|---|---|
| Alternative Names | Sperm-associated antigen 8 HSD-1 Sperm membrane protein 1 SMP-1 Sperm membrane protein BS-84 SPAG8 |
| Molecular Weight(MW) | 45 kDa |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
