Application
Western blot analysis of extract from T98G cells using SPARC Antibody.| Product Name | SPARC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ON, ABL, JTK7, ON, BM-40 |
| Product description | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.1) Kelm R. J. Jr., Mann K. G. Blood 75:1105-1113 (1990) Wewer U. M., Albrechtsen R., Fisher L. W., Young M. F., Termine J. D. Am. J. Pathol. 132:345-355 (1988) Kuhn C., Mason R. J. Am. J. Pathol. 147:1759-1769 (1995) |
| Immunogen | SPARC antibody was raised against a peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPARC |
|---|---|
| Alternative Names | ON ABL JTK7 ON BM-40 |
| Molecular Weight(MW) | 42 kDa |
| Tissue Specificity | This antibody detects endogenous level of total SPARC protein. |
| Entrez Gene | 6678 |
|---|---|
| Protein Accession | NP_003109.1 |
Application
Western blot analysis of extract from T98G cells using SPARC Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
