Application
Flow cytometric analysis of widr cells using SPARC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of SPARC Antibody in Y79 cell line lysates (35ug/lane)Application
Western blot analysis of SPARC using rabbit polyclonal SPARC Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.| Product Name | SPARC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, SPARC, ON |
| Product description | Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).1) Said,N., Oncogene 28 (39), 3487-3498 (2009) |
| Immunogen | This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SPARC |
|---|---|
| Alternative Names | SPARC Basement-membrane protein 40 BM-40 Osteonectin ON Secreted protein acidic and rich in cysteine SPARC ON |
| Molecular Weight(MW) | 35 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of widr cells using SPARC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of SPARC Antibody in Y79 cell line lysates (35ug/lane)
Application
Western blot analysis of SPARC using rabbit polyclonal SPARC Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
