Application
Immunohistochemistry of SPATA1 in mouse testis tissue with SPATA1 antibody at 2.5 μg/mL.Application
Immunofluorescence of SPATA1 in mouse testis tissue with SPATA1 antibody at 20 μg/mL.Application
Western blot analysis of SPATA1 in A20 cell lysate with SPATA1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SPATA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPATA1 Antibody: SP-2, SPAP1, Spermatogenesis-associated protein 1, Sperm-specific protein SP-2 |
| Product description | SPATA1 Antibody: SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3).1) Giesecke K, Hamann H, Stock KF, et al. Evaluation of SPATA1-associated markers for stallion fertility. Anim. Genet. 2009; 40:359-65. |
| Immunogen | SPATA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA1. The immunogen is located within amino acids 300 - 350 of SPATA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPATA1 |
|---|---|
| Alternative Names | SPATA1 Antibody: SP-2 SPAP1 Spermatogenesis-associated protein 1 Sperm-specific protein SP-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (72%) |
| Tissue Specificity | At least two isoforms of SPATA1 are known to exist; this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members. |
Application
Immunohistochemistry of SPATA1 in mouse testis tissue with SPATA1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SPATA1 in mouse testis tissue with SPATA1 antibody at 20 μg/mL.
Application
Western blot analysis of SPATA1 in A20 cell lysate with SPATA1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPATA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPATA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
