SPATA16 is involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion. Defects in SPATA16 are a cause of globozoospermia; also called Round-headed spermatozoa.1) Dam, A.H., (2007) Am. J. Hum. Genet. 81 (4), 813-820.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPATA16.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SPATA16 antibody can be used for detection of SPATA16 by ELISA at 1:62500. SPATA16 antibody can be used for detection of SPATA16 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
SPATA16 antibody can be used for detection of SPATA16 by ELISA at 1:62500. SPATA16 antibody can be used for detection of SPATA16 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPATA16 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.