Application
Immunohistochemistry of SPATA3 in mouse testis tissue with SPATA3 antibody at 2.5 μg/mL.Application
Immunofluorescence of SPATA3 in mouse testis tissue with SPATA3 antibody at 20 μg/mL.Application
Western blot analysis of SPATA3 in 3T3 cell lysate with SPATA3 antibody at 1 μg/mL.| Product Name | SPATA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPATA3 Antibody: TSARG1, TSARG1, Spermatogenesis-associated protein 3, Testis and spermatogenesis cell-related protein 1 |
| Product description | SPATA3 Antibody: The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.1) Fu JJ, Lu GX, Li LY, et al. Molecular cloning for testis spermatogenesis cell apoptosis related gene TSARG1 and Mtsarg1 and expression analysis for Mtsarg1 gene. Yi Chuan Xue Bao 2003; 30:25-9. |
| Immunogen | SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3. The immunogen is located within the last 50 amino acids of SPATA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPATA3 |
|---|---|
| Alternative Names | SPATA3 Antibody: TSARG1 TSARG1 Spermatogenesis-associated protein 3 Testis and spermatogenesis cell-related protein 1 |
| Molecular Weight(MW) | Predicted: 21 kDa Observed: 26 kDa |
| Sequence Similarities | 2) Cat. No. 1223 - Caco-2 Cell Lysate |
| Tissue Specificity | At least three isoforms of SPATA3 are known to exist; this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members |
Application
Immunohistochemistry of SPATA3 in mouse testis tissue with SPATA3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SPATA3 in mouse testis tissue with SPATA3 antibody at 20 μg/mL.
Application
Western blot analysis of SPATA3 in 3T3 cell lysate with SPATA3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPATA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPATA3 Antibody is supplied in PBS containing 0.02% sodium azide. |
