Application
Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 μg/mL.Application
Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 μg/mL.Application
Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SPATA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPATA4 Antibody: SPEF1B, TSARG2, Spermatogenesis-associated protein 4, Testis and spermatogenesis cell-related protein 2 |
| Product description | SPATA4 Antibody: The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.1) Liu SF, Lu GX, Liu G, et al. Cloning of a full-length cDNA of human testis-specific spermatogenic cell apoptosis inhibitor TSARG2 as a candidate oncogene. Biochem. Biophys. Res. Commun. 2004; 319:32-40 |
| Immunogen | SPATA4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA4. The immunogen is located within amino acids 200 - 250 of SPATA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPATA4 |
|---|---|
| Alternative Names | SPATA4 Antibody: SPEF1B TSARG2 Spermatogenesis-associated protein 4 Testis and spermatogenesis cell-related protein 2 |
| Tissue Specificity | At least two isoforms of SPATA4 are known to exist; this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members |
Application
Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 μg/mL.
Application
Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1415 - Mouse Stomach Tissue Lysate |
|---|---|
| Application Notes | SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPATA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPATA4 Antibody is supplied in PBS containing 0.02% sodium azide. |
