Application
Immunohistochemistry of SPATA6 in mouse testis tissue with SPATA6 antibody at 2.5 μg/mL.Application
Immunofluorescence of SPATA6 in mouse testis tissue with SPATA6 antibody at 20 μg/mL.Application
Western blot analysis of SPATA6 in A20 cell lysate with SPATA6 antibody at 1 μg/mL.| Product Name | SPATA6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPATA6 Antibody: HASH, SRF1, SRF-1, Spermatogenesis-associated protein 6 |
| Product description | SPATA6 Antibody: The development of spermatogonial stem cells into spermatozoa, i.e., spermatogenesis, is a highly ordered process that requires multiple proteins, including some that are testis-specific or testis-specific isoforms of genes that are expressed in other tissues. One such protein is SPATA6: while its full-length isoform is expressed in multiple tissues including thymus, brain and testis, the shorter isoform is expressed only in testis. It has some homology to the motor domain of kinesin related proteins and with the C. elegans neural calcium sensor protein NCS-2.1) Venables JP. Alternative splicing in the testes. Curr. Opin. Genet. Dev. 2002; 12:615-9. |
| Immunogen | SPATA6 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SPATA6. The immunogen is located within amino acids 400 - 450 of SPATA6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPATA6 antibody can be used for detection of SPATA6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPATA6 |
|---|---|
| Alternative Names | SPATA6 Antibody: HASH SRF1 SRF-1 Spermatogenesis-associated protein 6 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | At least three isoforms of SPATA6 are known to exist; this antibody will detect only the longest two isoforms. SPATA6 antibody is predicted to not cross-react with other SPATA protein family members. |
Application
Immunohistochemistry of SPATA6 in mouse testis tissue with SPATA6 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SPATA6 in mouse testis tissue with SPATA6 antibody at 20 μg/mL.
Application
Western blot analysis of SPATA6 in A20 cell lysate with SPATA6 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | SPATA6 antibody can be used for detection of SPATA6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPATA6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPATA6 Antibody is supplied in PBS containing 0.02% sodium azide. |
