Application
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of SPCS3 antibody in mouse liver tissue lysates (35ug/lane)| Product Name | SPCS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Signal peptidase complex subunit 3, 34--, Microsomal signal peptidase 22/23 kDa subunit, SPC22/23, SPase 22/23 kDa subunit, SPCS3, SPC22 |
| Product description | SPCS3 is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.1) Clark,H.F., Genome Res. 13 (10), 2265-2270 (2003) |
| Immunogen | This SPCS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the C-terminal region of human SPCS3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | SPCS3 |
|---|---|
| Alternative Names | Signal peptidase complex subunit 3 34-- Microsomal signal peptidase 22/23 kDa subunit SPC22/23 SPase 22/23 kDa subunit SPCS3 SPC22 |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of SPCS3 antibody in mouse liver tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
