Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
SPDYE3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in A375 cell line lysates (35ug/lane).| Product Name | SPDYE3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Speedy protein E3, SPDYE3 |
| Product description | 1) Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) : |
| Immunogen | This SPDYE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-483 amino acids from the Central region of human SPDYE3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPDYE3 |
|---|---|
| Alternative Names | Speedy protein E3 SPDYE3 |
| Molecular Weight(MW) | 64 kDa |
Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
SPDYE3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in A375 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
