Application
Western blot analysis of SPG15 in K562 cell lysate with SPG15 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of SPG15 in Mouse Heart tissue with SPG15 antibody at 20 μg/mL.| Product Name | SPG15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPG15 Antibody: SPG15, FYVE-CENT, KIAA0321, Zinc finger FYVE domain-containing protein 26, FYVE domain-containing centrosomal protein |
| Product description | SPG15 Antibody: Hereditary spastic paraplegias (HSPs) are genetically and phenotypically heterogeneous disorders. Spastic paraplegia with thinning of the corpus callosum (ARHSP-TCC) is a relatively frequent form of complicated hereditary spastic paraplegia in which mental retardation and muscle stiffness at onset are followed by slowly progressive paraparesis and cognitive deterioration. SPG15 is the second gene known to be responsible for ARHSP-TCC in the Italian population. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. SPG15 encodes a protein containing a FYVE zinc finger binding domain which is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. SPG15 mRNA is widely distributed in human tissues, as well as in rat embryos, suggesting a possible role for this protein during embryonic development. SPG15 co-localizes partially with endoplasmic reticulum and endosome markers, suggesting a role in intracellular trafficking.1) Hughes CA, Byrne PC, Webb S, et al. SPG15, a new locus for autosomal recessive complicated HSP on chromosome 14q. Neurology 2001; 56:1230-3. |
| Immunogen | SPG15 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human SPG15. The immunogen is located within amino acids 1710 - 1760 of SPG15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SPG15 antibody can be used for detection of SPG15 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZFYVE26 |
|---|---|
| Alternative Names | SPG15 Antibody: SPG15 FYVE-CENT KIAA0321 Zinc finger FYVE domain-containing protein 26 FYVE domain-containing centrosomal protein |
| Molecular Weight(MW) | Predicted: 280 kDa Observed: 260 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | Multiple isoforms of SPG15 are known to exist. |
Application
Western blot analysis of SPG15 in K562 cell lysate with SPG15 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of SPG15 in Mouse Heart tissue with SPG15 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | SPG15 antibody can be used for detection of SPG15 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPG15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPG15 Antibody is supplied in PBS containing 0.02% sodium azide. |
