Application
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.| Product Name | SPIB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPIB, SPI-B |
| Product description | SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).1) Geng, C.D. (2005) J. Biol. Chem. 280 (52), 43264-43271. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPIB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SPIB antibody can be used for detection of SPIB by ELISA at 1:62500. SPIB antibody can be used for detection of SPIB by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPIB |
|---|---|
| Alternative Names | SPIB SPI-B |
| Molecular Weight(MW) | 19 kDa |
Application
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | SPIB antibody can be used for detection of SPIB by ELISA at 1:62500. SPIB antibody can be used for detection of SPIB by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPIB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
