Application
Immunohistochemistry of SPINK2 in mouse testis tissue with SPINK2 antibody at 2.5 μg/mL.Application
Immunofluorescence of SPINK2 in mouse testis tissue with SPINK2 antibody at 20 μg/mL.Application
Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SPINK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPINK2 Antibody: HUSI-II, Serine protease inhibitor Kazal-type 2, Acrosin-trypsin inhibitor |
| Product description | SPINK2 Antibody: Human serine proteinase inhibitor Kazal-type 2 (SPINK2) is required for maintaining normal spermatogenesis and potentially regulates serine protease-mediated apoptosis in male germ cells. It contains a typical kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. SPINK2 plays a role in the pathogenesis of hereditary and chronic pancreatitis.1) Lee B, Park I, Jin S, et al. Impaired spermatogenesis and fertility in mice carrying a mutation in the Spink2 gene expressed predominantly in testes. J. Biol. Chem. 2011; 286:29108-17 |
| Immunogen | SPINK2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of mouse SPINK2. The immunogen is located within the last 50 amino acids of SPINK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPINK2 |
|---|---|
| Alternative Names | SPINK2 Antibody: HUSI-II Serine protease inhibitor Kazal-type 2 Acrosin-trypsin inhibitor |
| Molecular Weight(MW) | Predicted: 15 kDa Observed: 18 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (100%) |
| Tissue Specificity | SPINK2 antibody is predicted to not cross-react with other SPINK family members. |
| Entrez Gene | 69982 |
|---|---|
| Protein Accession | NP_001276693.1 |
Application
Immunohistochemistry of SPINK2 in mouse testis tissue with SPINK2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SPINK2 in mouse testis tissue with SPINK2 antibody at 20 μg/mL.
Application
Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPINK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPINK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
