Application
Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.Application
Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.Application
Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.| Product Name | SPINSTER Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPINSTER Antibody: LAT, nrs, SPIN1, SPINL, HSpin1, PP2030, PP20300, Protein spinster homolog 1 |
| Product description | SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1). SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2). The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1,3).1) Saier MH Jr, Beatty JT, Goffeau A, et al. The major facilitator superfamily. J. Mol. Microbiol. Biotechnol. 1999; 1:257-79. |
| Immunogen | SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER. The immunogen is located within the first 50 amino acids of SPINSTER. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPNS1 |
|---|---|
| Alternative Names | SPINSTER Antibody: LAT nrs SPIN1 SPINL HSpin1 PP2030 PP20300 Protein spinster homolog 1 |
| Molecular Weight(MW) | Predicted: 58 kDa Observed: 56 kDa |
| Sequence Similarities | 3) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins. |
| Entrez Gene | 83985 |
|---|---|
| Protein Accession | NP_001135920 |
Application
Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.
Application
Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.
Application
Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPINSTER antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SPINSTER antibody is supplied in PBS containing 0.02% sodium azide. |
