Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | SPO11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Meiotic recombination protein SPO11, Cancer/testis antigen 35, CT35, SPO11 |
| Product description | Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This SPO11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-326 amino acids from the C-terminal region of human SPO11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPO11 |
|---|---|
| Alternative Names | Meiotic recombination protein SPO11 Cancer/testis antigen 35 CT35 SPO11 |
| Molecular Weight(MW) | 45 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
