Application
Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 μg/mL.Application
Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 μg/mL.Application
Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SPP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPP1 Antibody: OPN, BNSP, BSPI, ETA-1, OPN, PSEC0156, Osteopontin, Bone sialoprotein 1, SPP-1 |
| Product description | SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).1) Heinegard D, Andersson G, and Reinholt FP. Roles of osteopontin in bone remodeling. Ann. N.Y. Acad. Sci.1995; 760:213-22. |
| Immunogen | SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1. The immunogen is located within amino acids 50 - 100 of SPP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPP1 |
|---|---|
| Alternative Names | SPP1 Antibody: OPN BNSP BSPI ETA-1 OPN PSEC0156 Osteopontin Bone sialoprotein 1 SPP-1 |
| Molecular Weight(MW) | Predicted: 36 kDa Observed: 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (94%) |
| Tissue Specificity | SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist. |
| Entrez Gene | 6696 |
|---|---|
| Protein Accession | NP_001238759 |
Application
Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 μg/mL.
Application
Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 μg/mL.
Application
Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1310 - Human Bladder Tissue Lysate |
|---|---|
| Application Notes | SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SPP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
