Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | SPP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPP1, OPN, BNSP, BSPI, ETA-1 |
| Product description | The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis.1) Iwata, M., et al., (2004) Blood 103 (12), 4496-4502. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SPP1 antibody can be used for detection of SPP1 by ELISA at 1:1562500. SPP1 antibody can be used for detection of SPP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPP1 |
|---|---|
| Alternative Names | SPP1 OPN BNSP BSPI ETA-1 |
| Molecular Weight(MW) | 33 kDa |
Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SPP1 antibody can be used for detection of SPP1 by ELISA at 1:1562500. SPP1 antibody can be used for detection of SPP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
